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KMID : 0880220080460040422
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Journal of Microbiology
2008 Volume.46 No. 4 p.422 ~ p.428
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The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export
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Moon Dong-Ge-Ra-Mi
Cho Hyun-Jin
Yoon Jin-Ho
Bae Jin-Ah
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Abstract
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We have isolated Gle1 homologue (named as spgle1) as a partial multicopy suppressor of the synthetic lethality of rae1-167 elf1-21 in fission yeast Schizosaccharomyces pombe. The spgle1 is also able to complement partially temperature-sensitive phenotype of rae1-167 only at a lower restrictive temperature. The spgle1 gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spgle1 gene is essential for vegetative growth and functional Gle1-GFP protein is localized mainly in NPC. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of spgle1 is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast.
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KEYWORD
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mRNA export, Schizosaccharomyces pombe, spgle1
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